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Rat IgG2b Kappa Isotype Control Monoclonal Clone LTF2 APC Ready To Use from Innovative Research is a flow cytometry isotype control, buffered in PBS with 0.05% Proclin300, 1% BSA. This isotype control has been specifically
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R&D Systems
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Image Search Results
Journal: Cell reports
Article Title: Targeting TREM2 on tumor-associated macrophages enhances immunotherapy.
doi: 10.1016/j.celrep.2021.109844
Figure Lengend Snippet: Figure 4. Fc effector-enhanced anti-TREM2 mAb therapy reverses anti-PD-1 resistance (A) Frequency of live TREM2+ target cells following co-culture with mouse BMDMs and varying amounts of anti-TREM2-WT (mouse immuno- globulin G2a [IgG2a]) or anti-TREM2 (afucosylated mouse IgG2a) (n = 4/group). Samples were com- bined from two independent experiments. (B) ADCC reporter cell fluorescence following co- culture with or ADCC reporter cells and TREM2+- expressing target cells and varying amounts of anti-TREM2-WT, anti-TREM2, or appropriate iso- types (n = 2/group). (C) Tumor growth of EMT6-tumor bearing animals treated with 5 mg/kg of isotype or anti-TREM2. Vertical dotted lines indicate days when mAb dosing occurred (n = 10/group). (D) Violin plots of intratumoral normalized TAMs as a frequency of CD45+ cells from isotype- and anti- TREM2-treated EMT6 tumor-bearing mice 24, 72, and 128 h after mAb treatment. Samples were combined from two independent experiments (experiment 1, n = 4–7/group; experiment 2, n = 10/group). Unless specified otherwise, all experiments were performed two or more times. For graphs with error bars, mean ± SEM is shown. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. See also Figure S4.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies anti-mouse XCR1 BV421 (clone ZET) Biolegend Cat # 148216; AB_2565230 anti-mouse F4/80 biotin (clone BM8) Biolegend Cat # 123106; AB_893501 Streptavidin BV510 Biolegend Cat # 405234 anti-mouse CD64 BV605 (clone X54-5/7.1) Biolegend Cat # 139323; AB_2629778 anti-mouse CD11c BV650 (clone N418) Biolegend Cat # 117339; AB_2562414 anti-mouse Ly-6C BV711 (clone HK1.4) Biolegend Cat # 128037; AB_2562630 anti-mouse CD90.2 BV785 (clone 30-H12) Biolegend Cat # 105331; AB_2562900 anti-mouse NKp46 BV785 (clone 29A1.4) Biolegend Cat # 137637; AB_2734201 anti-mouse/human CD45R/B220 BV785 (clone RA3-6B2) Biolegend Cat # 103246; AB_2563256 anti-mouse/human CD11b AF488 (clone M1/70) Biolegend Cat # 101217; AB_389305 anti-mouse CD45 PerCP/Cy5.5 (clone 30-F11) Biolegend Cat # 103132; AB_893340 anti-mouse Ly6G PE-e610 (clone 1A8-Ly6g) Invitrogen Cat # 61-9668-82; AB_2574679 anti-mouse CD24 PE/Cy7 (clone M1/69) Biolegend Cat # 101822; AB_756048 anti-mouse MHC-II AF700 (clone M5/ 114.15.2) Biolegend Cat # 107622; AB_493727 anti-mouse CD40 PE (clone 3/23) Biolegend Cat # 124610; AB_1134075 anti-mouse CD86 PE (clone GL-1) Biolegend Cat # 105008; AB_313151 Rat IgG2a, k PE isotype control PE (clone RTK2758) Biolegend Cat # 400508; AB_326530 anti-mouse/human TREM2 APC (clone 237920) R&D Systems Cat # FAB17291A;
Techniques: Co-Culture Assay, Expressing
Journal: Cell Reports Medicine
Article Title: Ablation of ERO1A induces lethal endoplasmic reticulum stress responses and immunogenic cell death to activate anti-tumor immunity
doi: 10.1016/j.xcrm.2023.101206
Figure Lengend Snippet:
Article Snippet:
Techniques: Control, Purification, Virus, Recombinant, CCK-8 Assay, Transfection, Protease Inhibitor, SYBR Green Assay, Saline, Lysis, Multiplex Assay, Cytotoxicity Assay, Cell Isolation, Enzyme-linked Immunosorbent Assay, Bicinchoninic Acid Protein Assay, Software